The present study describes the antimicrobial activity of Mollugo pentaphylla fruit extract against microorgnism. Bacillus substils, Staphylococcus aureus, Staphylococcus epidermis, Escherichia coli, Staphylococcus flexineria and Pseudomonas aeruginosa. For this purpose aqueous extract of fruit were prepared and tested by “Disc Diffusion Method”. As a result of this study it was found that the extract of fruit generally revealed anti?¢???microbial activity against both gram-positive bacteria (B. subtils, S. aureous and S. epidermis) and gram-negative bacteria (E. coli, S. flexineria and P.auriginosa).