Allergy is the immune disorder which occurs due to allergens present in environment. Once when the allergen enters the body, there arise over activation of basophils and mast cells by IgE antibody which results in high inflammatory response leading to varied allergic reactions like asthma, hay fever, eczema etc. Asp O 21 allergen present in common plant pathogen Aspergillus oryzae, which is considered as one of the causative agent of human atopic disorder, can serve as an important target for vaccine development. Hence this study was designed to predict potential B cell and T cell epitopes along with secondary structure, post translational modifications and protein secretion using immune-informatics tools.