Gallic acid (GA), rutin(R), quercitin (Q), ellagic acid (EA) and sitosterol(S) isolated from the methanolic extract of the leaves of Tectona grandis were formulated as 0.2 % ointment in emulsifying base and were evaluated for their wound healing activity. The animals were divided into eight groups of six animals each. Groups 1 used as control, group 2 used as a standard i.e. nitrofurazone ointment (0.2 % w/w), group 3 for emulsifying ointment base, groups 4,5,6, 7 and 8 were used for gallic acid,rutin ,quercitin, ellagic acid and sitosterol . The formulation containing the most active phytoconstituent was packed in air tight bottle and evaluated for itsstability. The ointment was stored at 00 ,300, 450 at different predetermined intervals i.e. 30, 60 and 90 days and was evaluated for its appearance, spreadability, texture, pH and phase separation. The animals treated with rutin showed complete healing on the 10th day, followed by gallic acid, Ellagic acid, Sitosterol, Quercitin. The formulation containing rutin was subjected to stability testing .There was no change in color, pH and no phase separation at 00 and 300 for 90 days. The pH of the ointment was found to be in the range of 5.95-6.36 .The spreadability of the formulation was found to lie between 33.92- 52.77 and the spreading time ranges between 18 sec to 28 sec. The texture of the ointment remained smooth through out the stability studies. The drug content of the ointment was found with in the range of 100%-97.07% at 300.
