A new, simple, precise, and accurate stability indicating RP-HPLC method for simultaneous estimation of Gatifloxacin and Ketorolac tromethamine as the bulk drug and in pharmaceutical dosage forms. Chromatographic separation of the drugs was performed on ACE C18 (250 x 4.6 mm; 5 μm particle size) analytical column as the stationary phase. The solvent system consisted of Water and methanol in the ratio of 60:40 (v/v) and the pH was adjusted to 5.2 with potassium dihydrogen phosphate as mobile phase. The flow rate was 1.0 mL/min, column temperature was 25°C and effluents were monitored at 280nm. The retention times of Gatifloxacin and Ketorolac tromethamine were 5.7 min and 4.6 min, respectively. The method had an acceptable precision and accuracy. The method had the potential to quantify the selected drugs simultaneously from tablet dosage forms without any interference of the excipients. The selected drugs were also subjected to acid, base, oxidation, thermal and photolytic degradation studies. The degradation products obtained were well resolved from the drugs. As the method might efficiently separate the drugs from its degradation products, it can be used for stability indicating analysis.
