A simple, selective, precise, and stability-indicating high-performance thin-layer chromatographic (HPTLC) method has been developed and validated for analysis of Atorvastatin calcium and Metoprolol succinate in capsules. Both the drugs were separated on aluminum-backed silica gel 60 F254 plates with Toluene: methanol: ethyl acetate: glacial acetic acid (7: 1.5: 1: 0.5 v/v/v/v) as mobile phase. This system was found to give compact bands for Atorvastatin calcium and Metoprolol succinate (RF values 0.28 ± 0.1 and 0.58 ± 0.1, respectively). Densitometric analysis of Atorvastatin calcium and Metoprolol succinate was performed at 276 nm. Regression analysis data for the calibration plots were indicative of good linear relationships between response and concentration over the range 500–3000 ng per band for Atorvastatin calcium and 1000-6000 ng per band for Metoprolol succinate The correlation coefficients, r2 , were 0.9974 and 0.9927 for ATS and MET calibration curve, respectively. The method was validated as per ICH guidelines for accuracy, precision, LOD, LOQ and robustness. Atorvastatin calcium and Metoprolol succinate were subjected to acid, base, peroxide, heat and UV-induced degradation.
