A new Schiff base ligand is synthesized from 9,10-Phenanthrenequinone and 4-bromoaniline. The synthesized ligand is complexed with Cu(II) metal ions and its structural arrangements, geometry are confirmed by 13C NMR, EI Mass, FT-IR, EPR, Electronic spectra and molar conductance values. The electronic spectra confirms the octahedral geometry for Co(II) and Ni(II) complexes and square planar geometry for the Cu(II) complex which is further supported by EPR spectrum. Affinities of the metal complexes towards CT-DNA are studied in detail using absorption and emission spectral techniques. It has been revealed that the complexes could bind to calf thymus (CT)-DNA via intercalation mode. Anti bacterial activity of the complexes are carried out using disc diffusion method against various bacterial strains like Staphylococcus aureus, Salmonella typhi, Pseudomonas aerugenosa and Escherichia coli.