A simple visible spectrophotometric method is developed and validated for Pitofenone in its pharmaceutical dosage, using 2, 4 Di nitrophenyl hydrazine (DNPH) also known as Brady’s reagent. The maximum absorbance of Pitofenone is found to be at max l = 228nm.On reaction with Brady’s reagent in methanolic NaOH solution it forms orange color hydrazone derivative of Pitofenone at room temperature. The hydrazone derivative shows maximum absorbance at max l = 424nm.The solution obeys Beer’s law in the concentration range of 1-35 m g / mL .The coefficient correlation was 0.998 for the, linear curve equation Y=0.004x+0.007. From the average absorbance value of 0.074, analytical, statistical and optical parameters were calculated and found to be in the prescribed limits. An average recovery of the solution was made to 97.8%.The proton NMR spectrum of Pitofenone, DNPH and hydrazone derivative of Pitofenone were recorded to verify the possible mechanism and also to support the method.
