A simple, fast and precise simultaneous estimation method using Vierodt’s method has been developed for the simultaneous determination of gatifloxacin and ambroxol hydrochloride in pure and combined tablet dosage form. Shimadzu UV-1700 instrument was used and λmax of gatifloxacin and ambroxol hydrochloride was found to be 289 and 246nm, respectively using methanol and 0.1M sodium hydroxide in the ratio of 8:2. Gatifloxacin and ambroxol hydrochloride were found to be linear in the concentration of 2-10μg/ml and 5-15μg/ml, respectively at their wavelengths. Amount found for gatifloxacin and ambroxol hydrochloride was found to be 397.61 and 73.91mg/tablet, respectively. Percentage recovery range was found to be within 99.45-100.3% for gatifloxacin and 99.73-103.42% for ambroxol hydrochloride. The percentage RSD was found to be lower than 2% proves that the method is precise. The application of simultaneous equation method by UV spectroscopy can be employed for the estimation of gatifloxacin and ambroxol hydrochloride for routine analysis for a combination containing these two components in pure and tablet dosage forms.
