Metal chelates of Tb(III) with lawsone, juglone, phthiocol and plumbagin which are hydroxy derivatives of 1,4 naphthoquinone are prepared. The chemical composition of the chelates is obtained by microanalysis and TGA. The spectroscopic properties are studied with the help of IR, UV-visible spectroscopy. The morphological changes after chelation are studied by SEM photographs. The antibacterial activity of the ligands and chelates is examined against three bacterial strains. The thermal spectral and antibacterial properties are compared within isomeric pairs of chelates.