Andrographis paniculata Burm. F (Acanthaceae) is a potent medicinal plant in the Indian systems of medicine. Traditionally, the leaves are used as/in influenza, bronchitis, gonorrohea, cholera, fertility and antibacterial, anticancer, antidiabetic, antiinflammatory and antisnake venom etc. In the present study, the hydroalcohol extracts of the leaves of Andrographis paniculata was carried out by soxhlet method and their antibacterial activity study was made by disc diffusion method at different concentrations (5μl,10μl and 20μl) against different gram-positive (Staphylococcus aureus, Pseudomonas aeruginosa and Bacillus subtilis) and gram negative (Escherichia coli, Serratia marcescens and Proteus vulgaris). The antibiosis was also compared with standard antibiotics, Rifamycin and Amoxyclav. It was recorded with a remarkable inhibition ability of the leaf extract against the tested organisms and was also found better than amoxyclav at low dilutions.
