The objective of the study was to develop a simple, accurate, precise and rapid UV spectrophotometric, area under curve (AUC), method for simultaneous estimation of amoxicillin and carbocisteine from combined dosage form. The validation was carried out by using ICH guidelines for the determination of amoxicillin and carbocisteine by using 0.1N hydrochloric acid as the solvent in pharmaceutical dosage form. The proposed area under curve method involves the measurement of area at selected analytical wavelength ranges and performing the analysis using “Cramer’s rule and Matrix method” .The two analytical wavelengths ranges were used i.e. 224-232 nm and 237- 246 nm for estimation of amoxicillin and carbocisteine respectively. The linearity of the proposed method was found in the concentration range of 10- 100 μg /ml (r2= 0.9968) for amoxicillin and carbocisteine (r2=0.9965) respectively. The percentage mean recovery was found to be 100.04% for amoxicillin and 100.12 % for carbocisteine respectively. The method was statistically validated for its linearity, accuracy and precision as per ICH guidelines. Both intra and inter day variation showed less percentage (%) RSD values indicating high grade of precision of this method
