A simple, specific, accurate, and precise RP-HPLC method was developed and validated for the estimation of Olmesartan Medoximil, Metaprolol Tartarate in bulk drug and pharmaceutical dosage forms. A Xterra C18 column having 150 mm x 4.6 mm, 5 μm in isocratic mode, with mobile phase containing Phosphate buffer (pH 2.8 with Ortho phosphoric acid) : Acetonitrile [35 : 65, v / v] was used. The flow rate was 0.5 ml / min and effluents were monitored at 284 nm. Chromatogram showed peak at a retention time of 3.654 ± 0.008 min for Olmesartan Medoximil and 5.167 ± 0.008 min for Metaprolol Tartarate. The method was validated for system suitability, linearity, precision, accuracy, specificity, ruggedness, robustness, LOD and LOQ. Recovery of Olmesartan Medoximil, Metaprolol Tartarate was found to be in the range of 99.22 - 100.11 % and showing linearity in the range of 10-50 μg / ml. The LOD and LOQ for estimation of Olmesartan Medoximil and Metaprolol Tartarate were found to be 0.003 μg / ml, 0.09 μg / ml, and 0.012μg / ml , 0.3 μg / ml respectively. Proposed method can be successfully applied for the quantitative determination of Olmesartan Medoximil and Metaprolol Tartarate in bulk drug and pharmaceutical dosage forms.
