A validated HPLC method for simultaneous estimation of Emtricitabine and Tenofovir Disproxil Fumerate in pharmaceutical dosage forms. Chromatography was carried out on a C18 column [250mm, 4.6m, 5μm] using a mixture of methanol: phosphate Buffer (65:35 v/v) as the mobile phase at a flow rate of 1 ml/min. Detection was carried out by using PDA detector. The retention time of the drugs was 2.461 and 6.231 min for Emtricitabine and Tenofovir Disproxil Fumerate respectively. The method produced linear responses in the concentration range of 10 to 50μg/ml for both drugs. The LOD and LOQ values were found to be 0.00752, 0.00218ug/ml for Emtricitabine and The LOD and LOQ values were found to be 0.00851,0.0315ug/ml for Tenofovir Disproxil Fumerate. The method was validated for linearity, precision, accuracy, LOD &LOQ in accordance with ICH guidelines. The proposed method was found to be applicable for determination of the drug in tablet dosage forms.
