A simple, specific and accurate reverse phase high performance liquid chromatographic method was developed for the simultaneous determination Bromhexine and Cephalexin in pharmaceutical dosage form. The column used was ODS C18 (250mm x 4.6 mm, 5m) in isocratic mode, with mobile phase containing phosphate buffer and acetonitrile (45:55 v/v). The buffer is prepared by adding 1.42gm of sodium dihyrogen ortho phosphate in a 1000ml of volumetric flask add about 900ml of milli-Q water added and degas to sonicate and finally make up the volume with water then pH adjusted to 5.0 with dil. orthophosphoric acid solution. The flow rate was 1.0ml/ min and effluents were monitored at 254 nm. The retention times of Bromhexine and Cephalexin were found to be 2.661 min and 4.271 min, respectively. The linearity for Bromhexine and Cephalexin were in the range of 1-6 μg/ml and 62.5-375 μg/ml respectively. The recoveries of Bromhexine and Cephalexin were found to be 98.58% to 101.45% w/v and 98.13% to 101.51%w/v, respectively. The proposed method was validated and successfully applied to the estimation of Bromhexine and Cephalexin in combined tablet dosage forms.
