A simple, selective, rapid, and precise reverse phase HPLC method has been developed for the simultaneous estimation of gatifloxacin and ambroxol hydrochloride in pharmaceutical dosage form. A phenomenex Luna C18 column (250x4.6mm, 5μ) was used for the separation. The mobile phase was 0.02M dibasic ammonium phosphate buffer: acetonitrile (60:40%v/v) (Ph7) at a flow rate of 1.5ml/min with detection at 265nm. The retention time of gatifloxacin and ambroxol hydrochloride was 2.870 and 3.607 minutes respectively. The developed method was validated in term of accuracy, precision, specificity, system suitability, linearity, and robustness, limit of detection and limit of quantification. The proposed method is suitable for simultaneous determination of gatifloxacin and ambroxol hydrochloride in pharmaceutical dosage form
