This paper describes validated reversed phase high performance liquid chromatography (HPLC) method for the simultaneous determination of ramipril(RPL), telmisartan(TLM), and Hydrochlorothiazide((HTZ) in combined tablet dosage forms. The isocratic reverse phase HPLC analysis was done on a Merck C18 4.0 mm× 250 mm, column with mobile phase consisting of 0.1%phosphoric acid(pH adjusted to 2.5 with triethylamine) - acetonitrile(58:42,v/v) at a flow rate of 1 mL/min. Quantification was carried out using a photo-diode array UV detector at 210 nm. The developed analytical method was validated according to International Conference on Harmonization guidelines and the acceptance criteria for parameters like accuracy, precision, linearity, specificity and system suitability were met in all cases. The method is simple, precise, and sensitive, and hence applicable for simultaneous determination of RPL, TLM, and HTZ in pure powder and tablets.
