A simple, rapid and accurate high performance liquid chromatography method is described for simultaneous determination of ofloxacin and ornidazole from combined dosage form i.e. tablets. The separation of drug was achieved on Zorbax Eclipse C18 (250 x 4.6 mm i.d.) with 5 μ particle size, column showed most favorable chromatographic pattern over the other columns. The mobile phase consisted of a mixture of buffer and acetonitrile (65:35 % ( v/v)). The buffer was 0.03 M disodium hydrogen phosphate solution adjusted the pH 3.2 with orthophosphoric acid. The detection was carried out at wavelength 284 nm. The mixture of buffer of pH 3.2 and acetonitrile (65:35% v/v) was used as a diluent. The method was validated for system suitability, linearity, accuracy, precision, robustness, stability of sample solution. The method has been successfully used to analyze ofloxacin and ornidazole from combined dosage form i.e. tablets.
