The antagonistic bacterial isolates namely Bacillus subtilis 03 and Pseudomonas fluorescens 04 isolated and characterized from rhizosphere of healthy groundnut plants were utilized in the study for the production of nanoparticles possessing antagonistic action towards the crown rot pathogen Aspergillus niger. Initially nano silver ions were produced by the interaction of metal-filtrate solution using 1mM silver nitrate and culture filtrate. Later these silver ions - induced bacterial nanoparticles were removed and concentrated from the mixture, assayed for its antagonistic activity by seeded agar technique. Seed dressing of these bacterial nanoparticles were found to inhibit the growth of A.niger in groundnut. Size and shape of these nanoparticles were characterized by SEM studies.
