Two simple and sensitive visible spectrophotometric methods (A and B) have been developed for the determination
of anagrelide in pure and pharmaceutical dosage forms. Method A is based on the oxidation of 3-methyl
benzothiazololinone-2-hydrazone (MBTH) by ferric chloride followed by its coupling with anagrelide in acidic
medium forming a dark green colored chromogen with an absorption maximum at 618 nm. Method B is based on
the reaction of anagrelide with Folin-Ciocateu(FC) reagent under alkaline conditions forms an intensive blue
colored chromogen with an absorption maximum at 685 nm. Beer’s law is obeyed in the concentration range of 5-30
μg/ml for method A and 10-70 μg/ml for method B. These methods were successfully applied to pharmaceutical
dosage forms and no interference was observed from common pharmaceutical excipients and additives.
