Phytochemical investigation of the chloroform and ethyl acetate soluble parts of the aqueous-MeOH extract of the aerial parts of Crotalaria saharae Cosson collected from the region of Bechar in the South West of Algeria led to the isolation of Stigmasterol 1, Daucosterol 2, Diosmetin 3, Diosmin 4 and Trifolirhizin 5. The structures were established by spectral analysis including HRESI-MS, UV and 2D NMR experiments (COSY, NOESY, HSQC and HMBC) and comparison with literature data. To the best of our knowledge compounds 1, 2, 3, 4 and 5 were described for the first time from this endemic species.
