Repeated exposure or consumption of food contaminated with pesticide residues can cause various disorders for the human body, especially the immune and hormonal disturbances and the onset of certain cancers. The multirésiduel assay method of pesticides in blood by (GC-MS) Clarus® 600/560 DMS PerkinElmer® we developed allowed us to conduct a screening of the majority of pesticide residues used in Moroccan agriculture. The calibration range contains a mixture of the most used pesticides. The extraction was performed in a solid medium by C18 SPE cartridges. This method uses a capillary column of 30 meters type Supelco®, the oven temperature was programmed from 90 ° C to 290 ° C with a gradient of 10 ° C / min. Helium was used as carrier gas with a flow rate of 0.8ml / min. Phosalone was used as internal standard (IS). The calibration curves of different pesticides are linear with concentrations ranging between 10 and 70 ppb with satisfactory correlation coefficients (R2> 0.99) and CV <20% for all results. In these analytical conditions we analyzed blood samples of Endocrinology of the University Hospital of Rabat. The results showed that some patients are contaminated with pesticide residue; dimethoate and bifenthrin. The hypothesis of the involvement of these pesticides in the development of hormonal disorders in patients is very likely. This method may be expanded and supplemented to prove this causal link.
