Experiments were conducted to find out the screening of disease resistant variety against loose smut wheat of laboratory condition in during 2012-2013. I have screening 20 germplasm/variety each of 15 varieties infected from loose smut of wheat, and 5 germplasm/ varieties not infected from loose smut of wheat, which are respectively effect of different varieties N.H.-1012, HUW-468, WH – 147, PBW- 502, WG-377. And highest infected of different varieties are T.B.W.-396 (36%), H.D. - 4672 (36 %). I have obtained different results of pots condition in during period 2012-2013 each of 17 varieties infected from loose smut of wheat, and 3 germplasm/ varieties not infected from loose smut of wheat, which are respectively effect of different varieties N.H.-1012, PBW- 502, HUW-468, and highest infected of different varieties are R.R.-21 (Sonalika) (33%), HP-1209 (33%), TBW-396 (36%), HD- 4672 (33%). And I have obtained different results of field condition in during period 2013-2014, each of 17 varieties infected from loose smut of wheat, and 3 germplasm/ varieties not infected from loose smut of wheat, which are respectively effect of different varieties N.H.-1012, PBW- 502, HUW-468, and highest infected of variety was R.R.- 21(Sonalika) (33%).