Extracellular high activity endo-glucanase produced by Rhizopus oryzae PR 7 was used for the bioconversion of various agro wastes like orange peel, sugarcane bagasse, dried flower, water hyacinth and coconut shell. Among the agro wastes tested, saccharification percentage was highest in orange peel followed by sugar cane bagasse. The maximum amount of bioconversion was accomplished within 30-45 minutes of incubation. The optimum pH and temperature for such bioconversion was 7.0 and 33°C respectively. Highest rate of saccharification was found at a substrate concentration of 5mg/ml and there was a positive correlation between enzyme concentration and saccharification. The amount of glucose production was enhanced in presence of Mn2+ and after pre treatment with deionized water.
