A simple reversed-phase high-performance liquid chromatographic (RP-HPLC) method has been developed and validated for simultaneous determination of Domperidone (DOM) and Pantoprazole(PAN) in capsules. The drugs were separated on an analytical column, HiQ Sil C18V, 4.6mm x 250 mm, 5 μm (Kya Tech Japan). The mobile phase was a mixture of acetonitrile and 0.05M potassium di- hydrogen phosphate buffer ( pH 6.5adjusted with ortho phosphoric acid) in ratio of 50:50%v/v plus 0.05%TEA. UV detection was performed at 290 nm. Total chromatographic analysis time per sample was approximately 8 min with DOM, PAN and acetophenone (internal standard) eluting with retention times of 4.57, 5.58, and 7.05 min, respectively. Calibration plots were linear over the concentration ranges 1–10 μg mL-1 and 2–20 μg mL-1 for DOM and PAN respectively. The limit of detection (LODs) were 0.15 and 0.03 μg/ml and limit of quantification (LOQs) were 0.51 and 0.10 μg/ml for DOM and PAN respectively. The method was validated for accuracy, precision, specificity, linearity, robustness and sensitivity. The developed method was successfully used for quantitative analysis of DOM and PAN in Pantop-D capsules.
