A rapid, sensitive, precise and economical along with excessive resolution RP-High Performance Liquid Chromatographic method for determination of Amoxicillin and Carbocisteine has been developed. The chromatography system contain reverse phase C18 Column (250×4.6mm, 5μm) with a mixture of Acetonitrile: Water in ratio of 10:90 as a mobile phase. The pH 3 of mobile phase was adjusted by OPA, flow rate of mobile phase was 1.0 ml min-1. UV detection was performed at 220 nm and 20μl sample was injected. The method was validated as per ICH guideline. The retention time of Carbocisteine was 2.3 min and Amoxicillin was 5.1 min. The calibration cure was linear in the range of 10-50 and 5-25 μg/ml for Amoxicillin and Carbocisteine respectively. The percentage RSD for precision and accuracy of method was found to be less than 2 %. The lower limit of detection and limit of quantification was 0.003, 0.002 and 0.01, 0.007 ppm for Carbocisteine and Amoxicillin Respectively. Recovery from tablet was between 100.32, 101.26, and 101.35 %. The reported method is cost effective as compared to earlier method8 because a) Use of acetonitrile is much lesser which ultimately makes it cost effective method, b) Use of water, pH adjusted to 3 instead of buffer reduces the backpressure of system. c) Buffer is hazardous to Column life, d) Resolution is more, better for quantification of Amoxicillin and Carbocisteine from bulk as well dosage forms.
