A simple Reverse phase HPLC method has been developed for the quantitative estimation of Atenolol Related substance in pure drug. The quantification was carried out using stainless steel column C18 125 mm x 4.0 mm, 5 μm BDS Hypersil in Isocratic mode with mobile phase containing 1.0 g of Octane-1-Sulphonic acid sodium salt and 0.4 g of Tetra – n - butyl ammonium hydrogen Sulphate in a mixture of 20 volumes of Tetrahydrofuran, 180 volumes of methanol and 800 volumes of 3.4 g/L solution of Potassium dihydrogen phosphate and pH adjusted to 3 ± 0.2 using dilute ortho-phosphoric acid. The method was linear in the range of 1.6-3.2 μg /ml. The proposed method was found to be simple, precise, accurate, and reproducible for the estimation of Atenolol Related substance.
