Present study was aimed at isolation of promising cellulase producing fungus, its identification and optimization of cultural conditions for production of cellulolytic enzymes. The cellulololytic activity of these cultures was studied by standard CM-cellulose and congo red plate assay method. The study related to process development involves optimization of different fermentation conditions (physical and nutritional) towards enhancement of cellulolytic enzymes production. Solid state fermentation cultural conditions (physical and nutritional factors) for cellulolytic enzymes production by the isolated promising Aspergillus niger were optimized. One factor at a time (OFAT) strategy was used for the optimization of medium components. Cellulase production with Aspergillus niger was highest at temperature 28°C, pH-7.0, incubation time-72 hrs, salt-NaCl, nitrogen source-peptone and in presence order soma of substrate-Apple peel and common grass
