Pesticides are used for the increase yield of the crops, Rhizobia are bacteria which fixes the nitrogen by the formation of legumes. In this paper we had checked whether the pesticide affects the Rhizobium or not i.e. the whether the rhizobium is resistant to the particular pesticide (e.g. Monochrotophos). Chick pea i.e. Cicer arietinum is one example rhizobium leguminosarum had showed the result, i:e it is resistant to the pesticide monocrotophos in additionally we had taken the sequence of the chick pea compare with the sequence of the other sequence of the rhizobium we had codended that other sequence matched with the cicer arietinum i.e. Fixes the nitrogen taken the 2d & 3D structure.