Moxifloxacin Hydrochloride (MOX) is a fourth generation fluoroquinolone broad spectrum antibiotic agent used in conjunctivitis. Two simple, quick and economical methods ‘Zero Order UV-Spectrophotometric (Method I)’ and ‘First Order Derivative spectrophotometric (Method II)’ using Area Under Curve (AUC) technique have been developed for routine analysis of MOX in bulk and ophthalmic solution. In double RO water, MOX showed maximum absorbance at 288.2 nm. In method I, AUC between two wavelengths 279.0 nm and 296.4 nm were selected. In Method II, UV-spectrum of MOX was derivatized into first order and two wavelengths 289.4 nm and 305.6 nm were selected for determination of AUC. In both these methods, MOX obeyed linearity in the concentration range of 2 - 12 μg/mL (r2 > 0.99). Proposed methods were applied for ophthalmic solution and amounts of MOX estimated by method I and method II were found to be 99.97 ± 1.21 and 99.86 ± 1.82, respectively. Both these methods were validated statistically and by recovery experiments.
