Three new, simple, accurate, cost effective, safe, sensitive spectrophotometric methods have been developed for simultaneous estimation ofloxacin and ornidazole in tablet dosage form by using aqueous solution of 2.0 M sodium benzoate solution, as a hydrotropic agent. Sodium benzoate solution and additives of tablet did not interfere in analysis, as sodium benzoate did not show any absorbance above 300nm. In 2.0M sodium benzoate solution, ofloxacin and ornidazole shows maximum absorbance at wavelength 332 nm and 320 nm respectively and isobestic point was observed at 303nm. Beer’s law was obeyed in the concentration range 5-35 μg/ml for ofloxacin and 5- 40μg/ml for ornidazole. Method-I is based on simultaneous equation method and method II is based on determination of Q-value. Method-III involves estimation of both drugs by two wavelength method, where difference in absorbance at 332 and 307nm (difference was zero for ornidazole ) were used for estimation of ofloxacin and difference at 320 and 341nm(difference was zero for ofloxacin ) were used for estimation of ornidazole. Results of analysis for methods were tested and validated for various parameters according to ICH guidelines, hence can be adopted for the routine analysis of ofloxacin and ornidazole in tablet dosage form.
