UV Derivative Spectrophotometric methods for Method I -Q absorbance ratioof Ketoprofen (KETO), Methyl Paraben (MP) and Propyl Paraben, (PP) in Gel were formulated in the present work. UV spectrophotometric method was performed at 288nm, 308nm and 309 nm for KETO, MP and PP respectively and Isoabsorptive point - 298nm The linearity lies between 10-50ug/ml for Ketoprofen and 2-10 ug/ml for Methyl Paraben (MP) ,0.2-1.0 ug/ml for Propyl Paraben, (PP) for all the three methods. Method II In the Area under curve method preparation of standard stock solution was same as mentioned in method I from the overlain spectra 290.5 to 297.5 (KETO) and 297.5 to 303 (MP) , 306.5 to 312.5 (PP) were selected for analysis. The calibration curve for Keto ,MP,PP were prepared in the Conc. range as mentioned in method I at the selected wavelength range in Methanol and distilled water (50:50). The linearity lies between 10-50ug/ml for Ketoprofen and 2-10 ug/ml for Methyl Paraben (MP), 0.2- 1.0 ug/ml for Propyl Paraben, (PP) for all the three methods. The proposed method percentage recoveries for of KETO, MP and PP were found to be 100.02±1.5409,99.94% ±1.7891,99.97% and , 99.97%±0.7662 for this method I and 100.02 % ±1.659 ,99.99% ±1.112,100.00 % ±1.008Method II respectively in formulated Gel. The proposed methods are highly sensitive, precise and accurate and therefore can be used for its intended purpose. All the methods showed good reproducibility and recovery with % RSD less than 1. All method were found to be rapid, specific, precise and accurate and can be successfully applied for the routine analysis of in Ketoprofen (KETO), Methyl Paraben (MP) and Propyl Paraben, (PP) bulk and combined dosage form The various parameters, such as linearity, precision, accuracy, specificity, robustness, limit of detection and limit of quantitation were studied according to (ICH) International Conference on Harmonization guidelines
