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Production of amylase from fruit peel using Aspergillus Niger by Solid State Fermentation | Abstract
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Abstract

Production of amylase from fruit peel using Aspergillus Niger by Solid State Fermentation

Author(s): M. Sathish Kumar*, Sanjay Kumar Singh, G. Neelima, P. Rahini and M.R.K.Rao

Solid state fermentation holds tremendous potential for the production of the amylase by Aspergillus Niger. In the present study Aspergillus species was isolated from an onion sample from the local market and it was allowed to grow in a PDA plates. Then the inoculum for SSF was prepared by the extract of sweet lime peel. For amylase production through SSF, sweet lime peel, along with MSM medium were taken in conical flask and inoculated with Aspergillus spp. After 96 hours, the growth of Aspergillus spp was found to be maximum at 28°C and at pH 6.2. Amylase was harvested through phosphate buffer at 6.2 pH. Amylase produced showed the activity of 90 U/ml/min.