The present article implies an extracellular phytase production by Aspergillus niger under submerged and solid fermentation. Physical and Chemical conditions tested for optimal production of phytase using the single variable mode optimization technique. A considerable higher phytase production was obtained using rice bran as solid substrate (17.8 U/ml & 28.6 U/ml) at pH 5, on 4th day of incubation at 300C. It has been inferred from the results that glucose as best carbon source for phytase production with 29.5 U/ml (SMF) and 42.4U/ml (SSF) activity and ammonium nitrate as good nitrogen source enhanced production of phytase from Aspergillus niger upto 31.8 U/ml in SMF and 45.2 U/ml in SSF.
