Proteases are the most important industrial enzymes and comprise about 25% of commercial
enzymes in the world. Two third of the industrially produced proteases are from microbial
sources. The proteolytic fungi Aspergillus spp was isolated from soil contaminated with abattoir
waste and screened on skim milk agar medium for proteolytic activity. The enzyme was
optimized supplementation of cheese whey and other favorable conditions like pH, temperature
and incubation time to the medium. The optimized conditions for enzyme production were 5.0, 32
± 20C and 5 days intervals respectively. The fermentation medium was supplemented with
different nitrogen and carbon sources to improve the enzyme production. Among the sources
tested in the present study, soybean meal (1%) was proved the best nitrogen source whereas
glucose and rice flour were shown at 1.5% and 1% for enzyme production by Aspergillus spp
respectively.
