To evaluate the effect of the slime of Helix aspersaon acetic acid induced colitis in male Wistar rats, the slime of Helix aspersa was prepared by using the simplest method in heliciculture. Toxicity test was done by using DL50 guidelines. Wistar rats were divided into five groups (n=6). Seven days before induction of colitis, Group C and ACT received NaCl 0.9%; Group ASA+ACT received 5-aminosalisylic acid (5-ASA) 100 mg /kg. Group Slime and Slime+ACT received Helix aspersa slime 20 ml/Kg. Colitis was induced by trans-rectal administration of 4% acetic acid on the 8th day. Blood was withdrawn for C-Reactive Protein (CRP) analyses and all animals were sacrificed after seven days of colitis induction, the colon and the liver was dissected. Colon was analysed macroscopically and microscopically. Biochemical assessment of tissue total proteins and malondialdehyde (MDA) was done in liver tissue homogenate. Helix aspersa showed significant reduction in MDA, macroscopic and microscopic lesions showed improvement compared to Group ACT. The slime of Helix aspersa showed significant amelioration of experimentally induced colitis, which may be attributed to its anti-inflammatory and antioxidant property. In conclusion, treatment by the slime offered protection against colon inflammation, thus the slime is a good immune stimulant and could also contain natural antioxidant molecules.
