The objective of the present work is to prepare Mefenamic acid loaded bovine serum albumin nanoparticles by Desolvation technique. Drug-polymer dispersion was prepared under continuous mechanical stirring. Desolvating agent was added by means of two methods. In continuous addition method desolvating agent was added at the rate of 1ml/1min whereas in Intermittent addition method the desolvating agent was added at the rate of 1ml/5mins. Appearance of turbidity was considered as the end point. A cross-linking agent was added to stabilize the nanoparticles and stirring was continued for 12hrs.The obtained nano formulations were studied for characterization and evaluation parameters.Among the five formulations of continuous addition method and intermittent addition method the F3 formulations was found to be the best formulation with entrapment efficiency of 93.13% and 95.05% respectively, loading capacity of 32.04% and 32.3% respectively, mean particle diameter of 211.6nm and 208.7nm respectively and zeta potential value of -49.8mVand -52.8mV respectively. On comparison the intermittent addition method was concluded as the best method for the preparation of mefenamic acid nanoparticles because of its small mean particle diameter (208.7nm), zeta potential value of -52.8mV, higher drug entrapment efficiency (95.05%) and sustained drug release profile.
