Based on the market value and scarcity of culture media, screening of alternative media for the growth of fungi is found to be an important task for microbiologist in developing countries. In this study an attempt was made to test the possibility of using some cheaply available local materials (Sago, raw dried Palmyrah tuber flour, tubers of sweet potato and cassava) as alternative media for fungal growth. The sago and Palmyrah tuber flour were able to solidify without adding agar at the concentration of 10g / 100ml and 16g / 100ml respectively, within 25 minutes. Culture discs of Mucor sp., Penicillium sp., Fusarium sp. and Trichoderma sp. were transferred on the centre of the test media and the control media, potato dextrose agar (PDA). The diameters of fungal colony were measured at different time points. The results were subjected to analysis of variance (ANOVA) (P<0.05) followed by Tukey test. This study demonstrated that the sago and Palmyrah tuber media were suitable for the growth of all the tested fungi and the growth of Penicillium sp. on Palmyrah tuber medium was significantly higher when compared to PDA and sago medium. The results for Trichoderma sp. on sago medium and Mucor sp. on sago and Palmyrah tuber media were comparatively better than PDA after 24 hours incubation. However, with the prolonged incubation period the growth rate increases quickly in PDA than in tested media. Present study clearly shows the possibility of using cheap locally available sago and Palmyrah tuber media as an alternative media for PDA for the fungal growth. Further study is needed to elucidate
