The phytochemical analysis and antioxidant properties of ethyl acetate extracts of Gynandropsis pentaphyla was performed in the present study. Phytochemical study included detection of flavonoids, alkaloids, terpenoids, saponins, tannins, steroids, quinons, proteins and cardiac glycosides. The plant was collected from paddy fields near Chengalpattu, Tamil Nadu, India. Strong presence of Alkaloids, Terpenoids and Steroids was observed whereas proteins, saponins, flavonoids and cardio glycoside were present in less amounts. Tanins were conspicuous by their absence in the plant extract. Strong antioxidant activity was observed at 5 mg concentration of plant extract.