In this study, three LTB (Escherichia coli heat-labile toxin B subunit) transgenic tomato lines (#1-3) were grown in the pot to characterize their growth and development, and evaluate fruit and LTB subunit productivity. Generally, the growth and development of transgenic tomato lines are not significantly different with non-transgenic tomato cultivar, “311” (commercial cultivar as reference). All of them took 120 days to final harvest, and number of fruit is near equivalent. However, fruit quality characteristics are relatively different and line #3 has highest values of dry matter (6.02%), reducing sugar (2.51%) and degree Brix (6.40%). The vitamin C and acidity of all transgenic lines and the control are the same content. LTB subunit only expressed in two lines #1 and #3 with contents of 1.04% and 1.19% of total soluble protein, respectively.