Plant growth – promoting rhizobacteria (PGPR) are free living bacteria, isolated from the rhizosphere, which when applied to seeds or crops, enhanced the growth of the plant or reduce the damage from soil–borne plant pathogens. It had been estimated that more than 100 million tones of nitrogen, potash and phosphate – chemical fertilizers had been used annually in order to increase plant yield. The potential negative effect of chemical fertilizers on the global environment and the cost associated with production had lead to research with the objective of replacing chemical fertilizers with bacterial inoculants. Fluorescent Pseudomonas strains were isolated from the soil samples collected from Bhojia Institute of Life Sciences, Budh, Baddi. and these Pseudomonas strains showed fluorescent colour on Kings B medium under U.V light used for further studies, The maximum optical density (0.16) was observed at 30°C which depicts the maximum growth of the bacteria. At low temperatures and with the increase in the temperature the optical density decreases which indicates that the growth of bacteria decreases.