Pseudomonas aeruginosa is a leading causes of nosocomial infections and responsible for 10% of all hospital-acquired infections. P. aeruginosa nosocomial infections is generally difficult to killed because bacterial resistant mechanism. Increasing bacterial resistance to all antibiotics of different classes are often obtained and transmitted by conjugation, transformation, or plasmid transduction. The aimed was conducted to determine the antibiotic resistant patterns of Pseudomonas aeruginosa isolated from clinical samples in West Sumatera, Indonesia. 95 clinical data were collected from M. Djamil Hospital, Padang (Indonesia). Antibiotic susceptibility was determined on Mueller Hinton agar using the disc diffusion method according to the modified Kirby-Bauer technique. Plasmid isolation was done using a commercial plasmid isolation kit QIAprep® Spin Miniprep Kit. The resultant plasmids were separated using gel electrophoresis. In this study, 34 (35.79%) of P. aeruginosa isolates were resistant to three or more classes of antibiotics. The plasmid analysis revealed that sixteen multidrug resistant P. aeruginosa isolates had a plasmid. A isolate with single plasmid band 300bp, twelve of the isolates had a single plasmid band >1kbp and two isolates (PASw7 and PASp11) had two plasmids band > 1kbp.
