Regeneration of whole plant from cotyledon explants was developed for Aegle marmelos (L.) corr. This method involved organogenic calli formation in a MS-basal medium supplement with BAP (Benzyl Amino Purine) and Zeatin-6-furfurylamine (0.5-2.5mg/l) in combination with NAA (0.5mg/l) under dark conditions. The well developed calli later developed shoots when transferred to hormone free medium and under illumination. Developed shoots continued to grow in MS medium free of hormones and produced roots at the presence of IBA (0.5-3.0mg/l and BAP (1.0mg/l)).Rooted plants survived well under acclimatization.
