Date palm (Phoenix dactylifera L.) contains a wide array of flavonoids, but little is known about their antimicrobial effects. In this paper a phytochemical and biological study of flavonoids extracted from the male spathe grown in Touggourt, Algeria at flowering Stage is conducted. Analysis by TLC chromatography of the three flavonoids extracts(diethyl ether, Ethyl-Acetate and n-Butanol) indicate that the n-Butanol phase is rich in flavonoids compounds compared to other fractions. The antimicrobial activity was determined on the strains(Streptococcus sp, Staphylococcus sp, Escherichia coli, Pseudomonas species, Condida) using the disk diffusion method and all samples showed an inhibitory effect on the microorganisms
