Preliminary phytochemical studies of the leaves of C.aurantium and Gas chromatography –Mass spectrometry (GC – MS) analysis of its essential oil for the determination of the constituents. In the present study to perform the preliminary phytochemical studies and organoleptic characters by standard procedures. Chemical composition of leaf essential oil was determined by GC-MS analysis. Preliminary phytochemical screening on the leaves revealed the presence of flavanoids, carbohydrates, phytosterols, volatile oil, saponins, tannins, proteins terpenoids and absence of glycosides, alkaloids and fixed oil. GC-MS profile of the essential oil obtained by hydro distillation from the fresh leaves showed the presence of 35 compounds. The major compounds were Eucalyptol (1, 8 Cineole - 43.05%), Sabinene (16.65%), β-Linalool (15.25%), α-Terpineol (8.025), α-Pinene (1.34%), β-Myrcene (1.20%), 4- Terpineol (1.11%), β- Pinene (1.01%), D-Limonene (0.97%), O-Cymene (0.88%). Some variations were observed in the composition and percentage of constituents in the essential oil when compared to previous studies. This is may be due to climate change, soil, altitude and other conditions. GC-MS study revealed essential oil extracted from the fresh leaves possesses poly phenol compounds. Traditionally leaves were used as an anti –microbial and from that are assumed that poly-phenols present in the essential oil may be the reason for the activity. The essential oil may be a good candidate of potential therapeutic effects like antimicrobial activity against bacteria, virus, particularly to MRSA with resistance modifying property and stimulation of innate immunity.
