The present study was aimed at investigating the phytochemical bioactive compounds of the ethanolic extract of leaves of Centella asiatica (Family: Mackinlayaceae) and its antioxidative, AChE inhibitory and anti-diabetic activity which may ultimately lead to the finding of more effective agent for the treatment and management of Alzheimer Disease and Diabetes. For this purpose, phytochemical screening was done by variety of methods; Antioxidant activity was assessed by using the 1,1-diphenyl-2-picryl hydrazyl (DPPH) scavenging assay and measuring Super oxide radical scavenging activity; Anti-Alzheimer activity was assayed by quantifying acetyl cholinesterase (AChE) and butyrylcholinesterase (BChE) enzyme and Antidiabetic activity was investigated by measuring glucose in alloxan induced diabetic rat. There were presence of alkaloids, glycosides, steroids, flavonoids, tannins and reducing sugars; 50% scavenging (IC50) of DPPH and Superoxide were 40.4 and 109.57 μg/ml respectively; IC50 of AChE and BChE were 170 and 226 μg/ml respectively; Alloxan induced rat has 32.6%, 38.8% and 29.9% reduction of blood glucose level at 3rd hours after ingestion of 250mg/kg, 500mg/kg and 1000mg/kg of C. asiatica leaves extract respectively. C. asiatica was also tested for acute toxicity where no death or signs of toxicity even at the dose of 3000 mg/kg in rats was observed. The results of conducted study revealed that, the presence of the bioactive compounds, some of which possess an ideal structure for the scavenging of free radicals, could be responsible for the versatile medicinal properties of this plant including significant Antioxidant, Anti-Alzheimer and Antidiabetic activities.
