Ethanolic extract of Homalomena aromatica root was selected to evaluate their in vitro antioxidant potential. Antioxidant potential of the plant part was estimated by different in vitro antioxidant assay procedures and their EC50 values were calculated. Screening of phytochemical constituents and high performance thin layer chromatography and high performance liquid chromatography analysis of the ethanolic extract of H. aromatica were done for identification of some functional compounds in the plant. The ethanolic extract of H. aromatica exhibited maximum total antioxidant activity at 9 μg/ml and 20 μg/ml for all the seven models of free radical scavenging activity. At a concentration of 9 and 20 μg/ml of the plant extract, superior free radical scavenging activity was recorded. The extract showed the presence of phenolics and flavonoids; the reducing power of the extract increased linearly with concentration. The results of the present study showed that the crude ethanolic extract of H. aromatica contains high amount of phenolics and flavonoids which is also confirmed by HPTLC and HPLC analysis. In conclusion, the plant might be an alternative to the synthetic Antioxidants available in the market.
