Herbal medicines based on the plant Thespesia populnea (L.) used in the folk medicine of different cultures of India and some parts of world. In the present research, an attempt was made to extract some medicinally important compounds from the seeds of Thespesia populnea (L.) by soxhlet extraction n-butanol as a solvent. The investigation was carried out to determine the chemical components of Thespesia populnea (L.) seeds using Perkin-Elmer Gas Chromatography–Mass Spectrometry (GC-MS), while the mass spectra of the compounds found in the extract was matched with the National Institute of Standards and Technology (NIST) library. GC-MS analysis revealed the presence of six compounds. The compounds were identified by comparing their retention time and peak area with that of literature and by interpretation of mass spectra. The results of the study suggest that the n-butanol extract possesses anti-oxidant activity.
