Chemical studies on stem bark of Antidesma venosum used in phytotherapy were done in vitro. The stem bark was extracted by Soxhlet in absolute methanol, and partitioned using ethyl acetate, n-pentanol and distilled water to give fractions coded EAA, NPE and AQE, respectively. Phytochemical screening of crude methanol extract (CME) and EAA showed presence of alkaloids, saponins, tannins and flavonoids; Saponins and tannins in NPE and AQE. These fractions together with CME were screened for antimicrobial activity against Staphylococcus aureus, Salmonella typhi and Escherichia coli. The highest antimicrobial activity was observed with EAA and least with AQE. All fractions and extract however, exhibited some activity on tested microorganisms.
