The experimental work is carried out for evaluate the photochemical Analysis and antimicrobial activities of Aqueous, Chloroform, Carbontetrachloride, Ethylacetate, Ethanolleaf extracts of Ageratina altissimia plant. Photochemical screening of the plant extracts showed the presence of various compounds like Alkaloids, Flavanoids, Carbohydrates, fats & fixed oils, steroids. The antimicrobial screening was carried out by two Gram +ve (Staphylococcus aureus NCIM 2079, Bacillus subtilis NCIM 2063) and three Gram –ve (Escherichia. coliNCIM2065 Pseudomonas aeruginosa NCIM 2036KlebsiellaaerogenesNCIM 2098) bacteria and fungi (Candida albicans NCIM 3102, Aspergillus niger NCIM 1054)[1] using agar diffusion methods {disk diffusion method for Aqueous extract; well diffusion method for solvent extracts} for bacteria and poisoned food technique for fungi compared with standard drug. MICs of the crude extracts were determined by broth macrodilution method. All the microorganisms were markedly affected by these extracts. The chloroform extract exhibited the lowest MIC value against Staphylococcus aureus (125 μg/ml) and (150 μg/ml) against Aspergillus niger .Finally, conclude that the plant having natural antiseptic action.
