By means of chromatography-mass-spectrometry in lipophilic complex of Crataegus submollis Sarg leaves had been established 16 terpenoids (23.37%), 5 aromatic compounds (2.94%), 10 fatty acids (35.67%), 8 higher alkanes and their derivatives (37.66%). For lipophilic complex was detected antibacterial activity, we are used the test-strains of bacterial cultures: Staphylococcus aureus 25923, Escherichia coli 25922, Pseudomonas aeruginosa 27853, Proteus vulgaris 4636, Bacillus subtilis 6633, Candida albicans 885-663. According the results of experiment was proved that Staphylococcus aureus and Bacillus subtilis are highly sensitive to lipophilic complex, Proteus vulgaris - moderate sensitive